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KMID : 0357319930280020143
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Journal of the Korean Society for Microbiology
1993 Volume.28 No. 2 p.143 ~ p.152
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In vitro Effects of gamma-IFN and LPS on the anti-Rickettsia tsutsugamushi Action of Murine Peritoneal Macrophage
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이복수
최이천/전창덕/박석돈/김인재/정헌택/김익상/장우현
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Abstract
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Activation of macrophages is required for overcoming infection of a host with intracellular pathognes, and it has been reported that r-IFN and bumor necrosis facotor(TNF-α) or lipopolysacchride(LPS) activate macrohages to kill intracellular
parasites.
Activated macrophages form reactive nitrogen intermediates from L-arginine, which are essential in the induction of anti-intracellular pathogens. The authors investigated whether these intermediates inhibit the multiplication of the obligate
intracellular organism, Rickettsia tsutsugamushi. When macrophages treated with r-IFN plus LPS compared to treated with r-IFN or LPS only, NO production was markedly increased and the number of rikettsiae within infected macrophage was
considerably
reduced in R. tustsugamushiinfected urine peritoneal resident macrophages. To assess whether RNIs act directly or indirectly on the intracellular proliferation of R. tsutsugamushi, macrophages were incubated with the L-arginine analogue
NG-monomethyl-L-arginine(NGMMA), which can inhibit the generation of RNIs. The ability of NGMMA to inhibit the microbiostatic capacity of activated macrophages for R. tsutsugamushi correlated with inhibition of NO. These results show that
activation of
macrophages by r-IFN plus LPS induces the production of RNIs that have direct effects on the inhibition of multiplication of R. tsutsugamushi in vitro.
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